A Mass Spectrometric Solution to the Address Problem of Combinatorial Libraries

A Mass Spectrometric Solution to the Address Problem of Combinatorial Libraries

The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1994-04, Vol.264 (5157), p.399-402
Hauptverfasser: Brummel, Christopher L., Irene N. W. Lee, Zhou, Ying, Benkovic, Stephen J., Winograd, Nicholas
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Amino acids
Biosynthesis
Chemicals
Chemistry
Exact sciences and technology
Image Processing, Computer-Assisted
Imaging
Ions
Isomers
Libraries
Mass
Mass spectrometry
Mass Spectrometry - methods
Microspheres
Molecular ions
Molecular Sequence Data
Molecular Weight
Molecules
Oligopeptides - chemistry
Organic chemistry
Peptides
Polystyrenes
Reactivity and mechanisms
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Beschreibung
Zusammenfassung:The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.8153627