Molecular cloning and nucleotide sequence of chicken avidin-related genes 1-5

Using avidin cDNA as a hybridisation probe, we detected a gene family whose putative products are related to the chicken egg-white avidin. Two overlapping genomic clones were found to contain five genes (avidin-related genes 1 - 5, avr1 - avr5), which have been cloned, characterized and sequenced. A...

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Veröffentlicht in:European journal of biochemistry 1994-03, Vol.220 (2), p.615-621
Hauptverfasser: Keinanen, R.A, Wallen, M.J, Kristo, P.A, Laukkanen, M.O, Toimela, T.A, Helenius, M.A, Kulomaa, M.S
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Sprache:eng
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Zusammenfassung:Using avidin cDNA as a hybridisation probe, we detected a gene family whose putative products are related to the chicken egg-white avidin. Two overlapping genomic clones were found to contain five genes (avidin-related genes 1 - 5, avr1 - avr5), which have been cloned, characterized and sequenced. All of the genes have a four-exon structure with an overall identity with the avidin cDNA of 88-92%. The genes appear to have no pseudogenic features and, in fact, two of these genes have been shown to be transcribed. The putative proteins share a sequence identity of 68-78% with avidin. The amino acid residues responsible for the biotin-binding activity of avidin and the bacterial biotin-binding protein, streptavidin, are highly conserved. Since avidin is induced in both a progesterone-specific manner and in connection with inflammation, these genes offer a valuable tool to study complex gene regulation in vivo.
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1994.tb18663.x