Nucleotide sequence corrections of the uidA open reading frame encoding β-glucuronidase

Part of the open reading frame of uidA, encoding β-glucuronidase, was sequenced and two differences were found with the previously reported nucleotide sequence [Jefferson et al., Proc. Natl. Acad. Sci. USA 83 (1986) 8447–8451]. One is a silent mutation, the other results in the Glu 279 → Gln substit...

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Veröffentlicht in:	Gene 1994-01, Vol.138 (1), p.259-260
Hauptverfasser:	Schlaman, Helmi R.M., Risseeuw, Eddy, Franke-van Dijk, Marry E.I., Hooykaas, Paul J.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular - methods Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Genes. Genome Glucuronidase - genetics GUS intron Molecular and cellular biology Molecular genetics Molecular Sequence Data nt reporter gene Open Reading Frames Point Mutation Polymerase Chain Reaction - methods transgenic gene expression
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Beschreibung
Zusammenfassung:	Part of the open reading frame of uidA, encoding β-glucuronidase, was sequenced and two differences were found with the previously reported nucleotide sequence [Jefferson et al., Proc. Natl. Acad. Sci. USA 83 (1986) 8447–8451]. One is a silent mutation, the other results in the Glu 279 → Gln substitution.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(94)90820-6