An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody
A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase c...
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| Veröffentlicht in: | Journal of immunological methods 1985, Vol.82 (2), p.225-231 |
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| container_title | Journal of immunological methods |
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| creator | Dao, M L |
| description | A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones. |
| doi_str_mv | 10.1016/0022-1759(85)90354-0 |
| format | Article |
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This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.</description><identifier>ISSN: 0022-1759</identifier><identifier>EISSN: 1872-7905</identifier><identifier>DOI: 10.1016/0022-1759(85)90354-0</identifier><identifier>PMID: 2413128</identifier><identifier>CODEN: JIMMBG</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>alkaline phosphatase ; Alkaline Phosphatase - analysis ; Antibodies, Bacterial - analysis ; Antigen-Antibody Complex - analysis ; Applied sciences ; Bacterial Proteins - immunology ; Collodion ; ELISA ; Evaluation Studies as Topic ; Exact sciences and technology ; Immunoenzyme Techniques ; nitrocellulose ; Other techniques and industries ; recombinant antigen ; Recombinant Proteins - immunology ; Staining and Labeling - methods ; Streptococcus mutans - immunology</subject><ispartof>Journal of immunological methods, 1985, Vol.82 (2), p.225-231</ispartof><rights>1985</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-afe40ca7b73f782fe12f19f1ee040d4399b0bc94d4467b521576d718cff793653</citedby><cites>FETCH-LOGICAL-c301t-afe40ca7b73f782fe12f19f1ee040d4399b0bc94d4467b521576d718cff793653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0022-1759(85)90354-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7975466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2413128$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dao, M L</creatorcontrib><title>An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.</description><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - analysis</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Antigen-Antibody Complex - analysis</subject><subject>Applied sciences</subject><subject>Bacterial Proteins - immunology</subject><subject>Collodion</subject><subject>ELISA</subject><subject>Evaluation Studies as Topic</subject><subject>Exact sciences and technology</subject><subject>Immunoenzyme Techniques</subject><subject>nitrocellulose</subject><subject>Other techniques and industries</subject><subject>recombinant antigen</subject><subject>Recombinant Proteins - immunology</subject><subject>Staining and Labeling - methods</subject><subject>Streptococcus mutans - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EKtvCPwDJB4TKITBOnDjuAamq-KhUiQucLcce77ok9hI7Rf33ON3VHpEs-TDPvJ55TMgbBh8ZsO4TQF1XTLTysm8_SGhaXsEzsmG9qCshoX1ONifkJTlP6R4AGHRwRs5qzhpW9xvy9zpQP-3n-ICWTph30dLoqA7ZbzFQixlN9jHQcoLPczQ4jssYE17R20CTzwtNWfvgw_apcfytRx-Q7ncx7Xc664TUxHC_bHUuT6zBQ7SPr8gLp8eEr4_3Bfn19cvPm-_V3Y9vtzfXd5VpgOVKO-RgtBhE40RfO2S1Y9IxROBgeSPlAIOR3HLeiaGtWSs6K1hvnBOy6drmgrw_5JYV_yyYspp8WnfQAeOSlOiavmNCFJAfQDPHlGZ0aj_7Sc-PioFafatVplplqr5VT74VlLa3x_xlmNCemo6CS_3dsa6T0aObdTA-nTAhRcu7rmCfDxgWFw8eZ5WMx2DQ-rl8gLLR_3-Of7VCnZA</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Dao, M L</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody</title><author>Dao, M L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-afe40ca7b73f782fe12f19f1ee040d4399b0bc94d4467b521576d718cff793653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - analysis</topic><topic>Antibodies, Bacterial - analysis</topic><topic>Antigen-Antibody Complex - analysis</topic><topic>Applied sciences</topic><topic>Bacterial Proteins - immunology</topic><topic>Collodion</topic><topic>ELISA</topic><topic>Evaluation Studies as Topic</topic><topic>Exact sciences and technology</topic><topic>Immunoenzyme Techniques</topic><topic>nitrocellulose</topic><topic>Other techniques and industries</topic><topic>recombinant antigen</topic><topic>Recombinant Proteins - immunology</topic><topic>Staining and Labeling - methods</topic><topic>Streptococcus mutans - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dao, M L</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dao, M L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1985</date><risdate>1985</risdate><volume>82</volume><issue>2</issue><spage>225</spage><epage>231</epage><pages>225-231</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2413128</pmid><doi>10.1016/0022-1759(85)90354-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of immunological methods, 1985, Vol.82 (2), p.225-231 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76386177 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | alkaline phosphatase Alkaline Phosphatase - analysis Antibodies, Bacterial - analysis Antigen-Antibody Complex - analysis Applied sciences Bacterial Proteins - immunology Collodion ELISA Evaluation Studies as Topic Exact sciences and technology Immunoenzyme Techniques nitrocellulose Other techniques and industries recombinant antigen Recombinant Proteins - immunology Staining and Labeling - methods Streptococcus mutans - immunology |
| title | An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody |
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