An improved method of antigen detection on nitrocellulose: In situ staining of alkaline phosphatase conjugated antibody

A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase c...

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Veröffentlicht in:	Journal of immunological methods 1985, Vol.82 (2), p.225-231
1. Verfasser:	Dao, M L
Format:	Artikel
Sprache:	eng
Schlagworte:	alkaline phosphatase Alkaline Phosphatase - analysis Antibodies, Bacterial - analysis Antigen-Antibody Complex - analysis Applied sciences Bacterial Proteins - immunology Collodion ELISA Evaluation Studies as Topic Exact sciences and technology Immunoenzyme Techniques nitrocellulose Other techniques and industries recombinant antigen Recombinant Proteins - immunology Staining and Labeling - methods Streptococcus mutans - immunology
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Zusammenfassung:	A biological stain for alkaline phosphatase was applied to detect immune complexes immobilized on nitrocellulose. This method was sensitive and useful for in situ staining of antigens, in dot-immunobinding assays and immunoblots. In addition to its sensitivity, the staining of alkaline phosphatase conjugated antibody was also highly stable, and allowed a permanent record of original immunochemical data. A rapid and simple method is also described using this technique for screening translation products of recombinant clones.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(85)90354-0