Entry into S phase is inhibited in human fibroblasts by rat liver poly(A) + RNA

Entry into S phase is inhibited in human fibroblasts by rat liver poly(A) + RNA

Initiation of DNA synthesis was inhibited in human fibroblasts following microinjection with poly(A) + RNA derived from normal rat liver. After sucrose gradient sedimentation of the RNA, the inhibitory activity was found to be limited to two adjacent fractions. Dilution experiments suggest a minimum...

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Veröffentlicht in:Experimental cell research 1985-10, Vol.160 (2), p.544-549
Hauptverfasser: Lumpkin, C.K., McClung, J.Keith, Smith, James R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological and medical sciences
Cell cycle, cell proliferation
Cell physiology
Centrifugation, Density Gradient
DNA Replication - drug effects
Fibroblasts - cytology
Fibroblasts - drug effects
Fundamental and applied biological sciences. Psychology
Humans
Interphase - drug effects
Kinetics
Liver - analysis
Microinjections
Molecular and cellular biology
Poly A - pharmacology
Rats
RNA - pharmacology
RNA, Messenger
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Zusammenfassung:Initiation of DNA synthesis was inhibited in human fibroblasts following microinjection with poly(A) + RNA derived from normal rat liver. After sucrose gradient sedimentation of the RNA, the inhibitory activity was found to be limited to two adjacent fractions. Dilution experiments suggest a minimum abundance level of 0.015% for this mRNA(s). Studies on the kinetics of this inhibition indicate a reversible inhibition with a duration of approx. 10 h.
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(85)90201-0