Discovery of a γ-carboxyglutamic acid-containing protein in human spermatozoa

Here we describe the identification of a γ-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented...

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Veröffentlicht in:	FEBS letters 1985-10, Vol.190 (1), p.137-141
Hauptverfasser:	Soute, Berry A.M., Müller-Esterl, Werner, de Boer-van den Berg, Marian A.G., Ulrich, Magda, Vermeer, Cees
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Carboxyglutamic Acid - analysis amino acid composition Amino Acids - analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Carboxylase Chemical Phenomena Chemistry Chromatography, High Pressure Liquid Decarboxylation Fundamental and applied biological sciences. Psychology Glutamates - analysis Humans Male man Miscellaneous Proteins Proteins - isolation & purification Sperm spermatozoa Spermatozoa - analysis Vitamin K Warfarin γ-Carboxyglutamic acid
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Zusammenfassung:	Here we describe the identification of a γ-carboxyglutamic acid-containing protein in human spermatozoa. After thermal decarboxylation the protein is a good substrate for vitamin K-dependent carboxylase from various origins. A quick purification procedure for the decarboxylated protein is presented and in a preliminary characterization we have established its M r, (28000–30000) and its amino acid composition.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80444-0