Cytochemical study of K+-dependent p-nitrophenyl-phosphatase activity in the urinary bladder of Salamandra salamandra

The ultrastructural Na+-K+-ATPase localization in the salamander urinary bladder has been studied by the histochemical techniques of Ernst (1972) and Mayahara et al. (1980). Reaction products have been specially found on the basal and lateral membranes of granular and mitochondria-rich cells. The variable presence of artifacts, often accompanying Pb-capture phosphatase cytochemistry, is discussed. Additional data on the active ion transport have been obtained by using electrophysiological techniques.