Characterization of insoluble macromolecular Sn(II) complex and its application to the 99mTc labeling of human serum albumin-bearing mercapto groups

A chelating ion exchange resin containing aminophosphonic acid groups was used as a polymer matrix for the preparation of an insoluble macromolecular Sn(II)(R-Sn) complex. Sn(II), which strongly bound to the surface of the polymer matrix by chelation, retained the ability to reduce 99mTc in the R-Sn...

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Veröffentlicht in:Applied radiation and isotopes 1994, Vol.45 (1), p.41-47
Hauptverfasser: Nakayama, M., Terahara, T., Ikeda, R., Wada, M., Koiso, T., Kimura, Y., Harada, K., Sugii, A., Tomiguchi, S., Kojima, A., Hara, M., Nakajima, R., Ohyama, Y., Takahashi, M.
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Sprache:eng
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Zusammenfassung:A chelating ion exchange resin containing aminophosphonic acid groups was used as a polymer matrix for the preparation of an insoluble macromolecular Sn(II)(R-Sn) complex. Sn(II), which strongly bound to the surface of the polymer matrix by chelation, retained the ability to reduce 99mTc in the R-Sn complex. Human serum albumin-bearing-mercapto groups (HMA) was labeled with 99mTc at pH 2–3 using the R-Sn complex or SnCl 2 as reducing agent. The 99mTc-HMA labeled with the R-Sn complex resulted in a higher sustained level of radioactivity in the blood of mice than the 99mTc-HMA labeled with SnCl 2. These results suggest that the use of the R-Sn complex minimized Sn(II) contamination of the 99mTc labeling solution and can be used effectively as a reducing agent for 99mTc labeling of proteins containing sulfhydryl groups.
ISSN:0969-8043
1872-9800
DOI:10.1016/0969-8043(94)90146-5