Nitric oxide donor GEA 3162 inhibits endothelial cell-mediated oxidation of low density lipoprotein

The effect of a nitric oxide (NO) donor GEA 3162 on the endothelial cell (EC)-mediated oxidation of low density lipoprotein (LDL) was studied. In comparison to LDL incubated with EC without GEA 3162, the presence of GEA 3162 inhibited LDL oxidation by EC, as indicated by the following findings. (a)...

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Veröffentlicht in:	FEBS letters 1994-01, Vol.337 (2), p.179-183
Hauptverfasser:	Malo-Ranta, Ulla, Ylä-Herttuala, Seppo, Metsä-Ketelä, Timo, Jaakkola, Olli, Moilanen, Eeva, Vuorinen, Pauli, Nikkari, Tapio
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Aorta Atherosclerosis Biological and medical sciences Cells, Cultured DMEM, Dulbecco's modified Eagle's medium EC, endothelial cells Electrophoresis, Polyacrylamide Gel Endothelial cell Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology Humans Immunoblotting LDL, low density lipoprotein Lipoproteins, LDL - drug effects Lipoproteins, LDL - isolation & purification Lipoproteins, LDL - metabolism Lipoproteins, myelin LPDS, lipoprotein-deficient serum Macrophages, Peritoneal - drug effects Macrophages, Peritoneal - metabolism MDA, malondialdehyde Mice Nitric oxide Nitric Oxide - pharmacology NO, nitric oxide O2 −, superoxide anion Oxidation-Reduction Oxidized low density lipoprotein Proteins Rats SDS-PAGE, SDS-polyacrylamide gel electrophoresis SIN-1 SIN-1,3-morpholinosydnonimine-hydrochloride TBARS, thiobarbituric acid-reactive substances Triazoles - pharmacology
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Zusammenfassung:	The effect of a nitric oxide (NO) donor GEA 3162 on the endothelial cell (EC)-mediated oxidation of low density lipoprotein (LDL) was studied. In comparison to LDL incubated with EC without GEA 3162, the presence of GEA 3162 inhibited LDL oxidation by EC, as indicated by the following findings. (a) The degradation rate of LDL in macrophages was reduced to control levels. (b) The electrophoretic mobility of LDL decreased in a dose-dependent manner, (c) The concentrations of thiobarbituric acid-reactive substances and hydroperoxide-derived hydroxy fatty acids were lower. (d) The breakdown of apolipoprotein B was reduced. The results indicate that GEA 3162 prevents EC-mediated oxidation of LDL.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(94)80269-6