A modified colorimetric micro method for long-chain fatty acids and its application for assay of lipolytic enzymes

A modified colorimetric micro method for long-chain fatty acids and its application for assay of lipolytic enzymes

A modification of the Duncombe method for measurement of free fatty acids is presented. Substitution of diphenylcarbohydrazide for diethyldithiocarbamate color complexing agent increases the sensitivity of the method 4- to 6-fold. The absorbancy of the color produced is measured at 540 mμ. Applicati...

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Veröffentlicht in:Analytical biochemistry 1969-03, Vol.27 (3), p.387-396
Hauptverfasser: Mahadevan, S., Dillard, C.J., Tappel, A.L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Colorimetry
Fatty Acids - analysis
Hydrogen-Ion Concentration
Lipase - analysis
Liver - enzymology
Lysosomes - enzymology
Methods
Microchemistry
Phenylhydrazines
Phosphatidylcholines - analysis
Phospholipases - analysis
Rats
Spectrum Analysis
Time Factors
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Zusammenfassung:A modification of the Duncombe method for measurement of free fatty acids is presented. Substitution of diphenylcarbohydrazide for diethyldithiocarbamate color complexing agent increases the sensitivity of the method 4- to 6-fold. The absorbancy of the color produced is measured at 540 mμ. Applications of the method for the assay of pancreatic lipase, and rat liver lysosomal lipase and phospholipase are described.
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(69)90052-9