Lack of effect of GABA on [3H]leucine incorporation into a rat oviduct ribosomal system

A ribosomal system for [3H]leucine incorporation was isolated from the rat oviduct in order to study the possible effect of GABA on [3H]leucine incorporation during the estrous cycle. The system showed an absolute requirement for Mg2+ and about 50% dependence on an energy source. Optimal [3H]leucine...

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Veröffentlicht in:	Neurochemical research 1985-06, Vol.10 (6), p.789-796
Hauptverfasser:	ORENSANZ, L. M, AZUARA, C, FERNANDEZ, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids - metabolism Animals Biological and medical sciences Energy Metabolism Estrus Fallopian Tubes - metabolism Female Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - pharmacology In Vitro Techniques Leucine - biosynthesis Mammalian female genital system Morphology. Physiology Pregnancy Rats Rats, Inbred Strains Ribosomes - metabolism Vertebrates: reproduction
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Beschreibung
Zusammenfassung:	A ribosomal system for [3H]leucine incorporation was isolated from the rat oviduct in order to study the possible effect of GABA on [3H]leucine incorporation during the estrous cycle. The system showed an absolute requirement for Mg2+ and about 50% dependence on an energy source. Optimal [3H]leucine incorporation occurred under 3-6 mM Mg2+ and 100 mM K+ and was higher in diestrous-1 than in estrous or proestrous. GABA (10 mM) had no effect on [3H]leucine incorporation in any of the three estrous phases studied.
ISSN:	0364-3190 1573-6903
DOI:	10.1007/BF00964536