Determination of reducing sugars in the nanomole range with tetrazolium blue
Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2′,5,5′-tetraphenyl- 3,3′-dimethoxy 4,4′-biphenylene)ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreas...
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Veröffentlicht in: | Journal of biochemical and biophysical methods 1985-08, Vol.11 (2), p.109-115 |
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Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
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Online-Zugang: | Volltext |
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Zusammenfassung: | Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2′,5,5′-tetraphenyl- 3,3′-dimethoxy 4,4′-biphenylene)ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as little as 1 nmol of neutral sugars as well as 2-amino and
N-acetyl amino sugars. |
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ISSN: | 0165-022X 1872-857X |
DOI: | 10.1016/0165-022X(85)90046-6 |