A wasp venom mastoparan-induced polyphosphoinositide breakdown in rat peritoneal mast cells

The phospholipid metabolism of rat peritoneal mast cells stimulated with mastoparan, a secretagogue purified from wasp venom, was investigated. Mastoparan at 20 μg ml caused a rapid secretion of histamine. Mastoparan induced a transient decrease of phosphatidylinositol 4,5-bisphosphate on 32P labeli...

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Veröffentlicht in:	FEBS letters 1985-09, Vol.188 (2), p.363-366
Hauptverfasser:	Okano, Yukio, Takagi, Hajime, Tohmatsu, Toyohiko, Nakashima, Shigeru, Kuroda, Yoichiro, Saito, Kazuki, Nozawa, Yoshinori
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Bee Venoms - pharmacology Biological and medical sciences BSA, bovine serum albumin Calcium DG, diacylglycerol Fundamental and applied biological sciences. Psychology GTP, guanosine 5 '-triphosphate Histamine Release - drug effects In Vitro Techniques Inositol Phosphates - metabolism IP, inositol phosphate IP2, inositol bisphosphate Ip3, inositol trisphosphate Lipids Mast cell Mast Cells - drug effects Mast Cells - metabolism Mastoparan MCM, mast cell medium Other biological molecules PA, phosphatidic acid PC, phosphatidylcholine PE, phosphatidylethanolamine Peptides Peritoneal Cavity Phosphatidylinositols - metabolism Phosphoinositide PI, phosphatidylinositol PIP, phosphatidylinositol 4-phosphate PIP2, phosphatidylinositol 4,5-bisphosphate Rats Rats, Inbred Strains Wasp Venoms - pharmacology
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Zusammenfassung:	The phospholipid metabolism of rat peritoneal mast cells stimulated with mastoparan, a secretagogue purified from wasp venom, was investigated. Mastoparan at 20 μg ml caused a rapid secretion of histamine. Mastoparan induced a transient decrease of phosphatidylinositol 4,5-bisphosphate on 32P labeling and generation of a water-soluble degradation product, inositol trisphosphate on [ 3H]inositol labeling, suggesting the activation of phospholipase C upon stimulation.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80403-8