Quantitative microcomplement fixation tests using chicken anti-viral antibody extracted from egg yolk

Yolk immunoglobulins obtained from hens immunized with human rotavirus and two plant virus antigens were used in quantitative microcomplement fixation tests. Difficulties inherent in the use of avian complement were overcome by utilizing a mixture of the C1 component of chicken complement and guinea...

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Veröffentlicht in:	Journal of virological methods 1985-01, Vol.11 (3), p.217-223
Hauptverfasser:	van Regenmortel, M.H.V., Burckard, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral - analysis Antigens, Viral - analysis Biological and medical sciences Chickens - immunology Complement Fixation Tests Egg Yolk Female Fundamental and applied biological sciences. Psychology Immunoglobulins Microbiology microcomplement fixation plant viruses Plant Viruses - immunology rotavirus Rotavirus - immunology Species Specificity Techniques used in virology Tobacco Mosaic Virus - immunology Viral Envelope Proteins - analysis Virology yolk immunoglobulin
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Beschreibung
Zusammenfassung:	Yolk immunoglobulins obtained from hens immunized with human rotavirus and two plant virus antigens were used in quantitative microcomplement fixation tests. Difficulties inherent in the use of avian complement were overcome by utilizing a mixture of the C1 component of chicken complement and guinea-pig complement devoid of its own C1. The method is suitable for detecting small quantities of viral antigen and antibody and for detecting antigenic activity present on peptide fragments of viral proteins.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(85)90110-7