Phosphorothioates in pre-tRNAs can change the specificities of RNAses P or reduce the cleavage efficiencies

Several phosphorothioate-madified E coli and yeast pre-tRNAs were synthesized. If this modification included the phosphodiester at the RNase P cleavage site, two different effects were observed. With some pre-tRNAs the RNase P cleavage efficiency was severely reduced, whereas with other pre-tRNAs a...

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Veröffentlicht in:Biochimie 1993, Vol.75 (11), p.955-962
Hauptverfasser: Kahle, D, Küst, B, Krupp, G
Format: Artikel
Sprache:eng
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Zusammenfassung:Several phosphorothioate-madified E coli and yeast pre-tRNAs were synthesized. If this modification included the phosphodiester at the RNase P cleavage site, two different effects were observed. With some pre-tRNAs the RNase P cleavage efficiency was severely reduced, whereas with other pre-tRNAs a new reaction type for RNase P was observed. Unlike the previously studied base or ribose modifications, phosphorothioates resulted in aberrant cleavages at unmodified phosphodiesters. These new sites could be located in the 5′-flank or in the acceptor stem of the tRNA domain. Modified mutants of E coli pre-tRNA Tyr with different base pairs at the RNase P cleavage site were cleaved with reduced efficiencies, but no aberrant products were observed.
ISSN:0300-9084
1638-6183
DOI:10.1016/0300-9084(93)90145-I