Characterization of melatonin binding to the anteroventral and anterodorsal thalamic nuclei of the rat

Binding of 2-[ 125I]melatonin to the anteroventral and anterodorsal nuclei of the rat limbic thalamus was analyzed with in vitro autoradiography. In both nuclei the binding was of high affinity ( K d values 24–41 pM) and was completed with by nanomolar concentrations of 6-hydroxymelatonin. K d value...

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Veröffentlicht in:	European journal of pharmacology 1993-11, Vol.250 (1), p.161-163
Hauptverfasser:	Fredrik, O., Lindroos, C., Veilahti, Jukka, Leinonen, Lea M., Laakso, Maija-Liisa
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anterior thalamus Autoradiography Binding Sites Binding, Competitive Biochemistry and metabolism Biological and medical sciences Central nervous system Fundamental and applied biological sciences. Psychology In Vitro Techniques Iodine Radioisotopes Limbic system Male Melatonin Melatonin - analogs & derivatives Melatonin - metabolism Melatonin - pharmacology Pineal gland Pineal Gland - metabolism Rats Rats, Wistar Receptor autoradiography Thalamic Nuclei - metabolism Vertebrates: nervous system and sense organs
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Zusammenfassung:	Binding of 2-[ 125I]melatonin to the anteroventral and anterodorsal nuclei of the rat limbic thalamus was analyzed with in vitro autoradiography. In both nuclei the binding was of high affinity ( K d values 24–41 pM) and was completed with by nanomolar concentrations of 6-hydroxymelatonin. K d values were in the same range as those reported for other high-affinity binding sites in the brain. B max values were 0.68–2.99 fmol/mg protein. The results suggest that some effects of melatonin are mediated via the limbic thalamus.
ISSN:	0014-2999 1879-0712
DOI:	10.1016/0014-2999(93)90635-U