Succinate cytochrome c reductase in schistosomiasis: in vitro inhibition by some schistosomicidal drugs
Enzymes in mitochondria play an important role in biological oxidation and energy production. To understand the effect of schistosomiasis on these important processes, succinate cytochrome c reductase (SCR) from control and Schistosoma -infected mice was subjected for investigation. In this article,...
Gespeichert in:
Veröffentlicht in: | Journal of physiology and biochemistry 2010-12, Vol.66 (4), p.291-299 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Enzymes in mitochondria play an important role in biological oxidation and energy production. To understand the effect of schistosomiasis on these important processes, succinate cytochrome
c
reductase (SCR) from control and
Schistosoma
-infected mice was subjected for investigation. In this article, we report that SCR from
Schistosoma
-infected mouse showed a significant decrease in its
V
max
and
K
m
compared to control using both cytochrome
c
and 2,6-dichlorophenolindophenol as substrates. Furthermore, the kinetic studies of the purified SCR in the absence and presence of the schistosomicidal drugs praziquantel and
Commiphora
extract reveal that both drugs have an inhibitory action on the enzyme from the control and
Schistosoma
-infected mice and praziquantel changes the type of inhibition of SCR towards cytochrome
c
from mixed type in control to a competitive one in the case of the infection. |
---|---|
ISSN: | 1138-7548 1877-8755 |
DOI: | 10.1007/s13105-010-0035-5 |