Analysis of inactive X chromosome structure by in situ nick translation

Nick translation assays of fixed interphase female fibroblasts with tritiated nucleotides demonstrated a characteristic absence of label over sex chromatin. The chromatin bodies were nearly always peripheral in location and a ribbon of nick translatable DNA was detected between the sex chromatin and...

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Veröffentlicht in:	Chromosoma 1985, Vol.92 (3), p.209-213
Hauptverfasser:	DYER, K. A, RILEY, D, GARTLER, S. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Cells, Cultured Chromatin - ultrastructure DNA-Directed DNA Polymerase - metabolism Escherichia coli - enzymology Female Fibroblasts - cytology Fundamental and applied biological sciences. Psychology Humans Interphase Microscopy, Electron Molecular and cellular biology Molecular genetics Protein Biosynthesis Thymine Nucleotides - metabolism Translation. Translation factors. Protein processing Tritium X Chromosome - metabolism X Chromosome - ultrastructure
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Zusammenfassung:	Nick translation assays of fixed interphase female fibroblasts with tritiated nucleotides demonstrated a characteristic absence of label over sex chromatin. The chromatin bodies were nearly always peripheral in location and a ribbon of nick translatable DNA was detected between the sex chromatin and the nuclear envelope. High voltage electron microscopy indicated the possibility of a special nuclear envelope attachment region. The apparent resistance of sex chromatin to nick translation did not appear to be due to resistance to DNase I attack.
ISSN:	0009-5915 1432-0886
DOI:	10.1007/BF00348695