Effect of some anti-inflammatory drugs on FMLP-induced chemotaxis and random migration of rat polymorphonuclear leucocytes

The effect of indomethacin, acetyl salicylic acid and niflumic acid on the chemotaxis and random migration of rat polymorphonuclear leucocytes (PMN) was nvestigated with a modified Boyden chamber technique under various experimental conditions (two cell sources, administration of drugs in vivo or incubation in vitro, modulation of antichemotactic activity of sera obtained from animals with inflammatory reactions). Indomethacin and niflumic acid inhibited the chemotactic responsiveness of cells collected from the rat pleural cavity after induction of two types of acute inflammatory reactions. This action was dose-dependent and appeared after either in vivo administration of the drug or in vitro incubation of the cells with various concentrations of the drug. However, random migration was not significantly modified and acetyl salicylic acid had no effect under any of the conditions. The same drugs, acetyl salicylic excepted, inhibited the antichemotactic activity exhibited by sera obtained from animals with inflammatory reactions.