Implication of proteases in the respiration dependent inactivation of the lactose permease of E. coli

The lactose permease of E. coli becomes irreversibly inactivated during lactose transport under conditions of high respiratory activity. This inactivation is characterized by a decrease in the steady state of lactose accumulation, a decrease in the influx rate of lactose, and a decrease in the trans...

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Veröffentlicht in:	FEBS letters 1985-07, Vol.187 (1), p.38-42
Hauptverfasser:	Therisod, Hélène, Ghazi, Alexandre, Shechter, Emanuel
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences CCCP, carbonyl cyanide m-chlorophenylhydrazone Cell physiology E. coli Lactose permease Protein inactivation Serine protease Respiratory chain E. coli Lactose permeaseProtein inactivationSerine proteaseRespiratory chain Escherichia coli - enzymology Escherichia coli Proteins Fundamental and applied biological sciences. Psychology HQNO, 2-heptyl-4-hydroxyquinoline N-oxide Lactose - metabolism Membrane and intracellular transports Membrane Potentials Membrane Transport Modulators Membrane Transport Proteins - antagonists & inhibitors Molecular and cellular biology Monosaccharide Transport Proteins Oxygen Consumption Peptide Hydrolases - metabolism Phenylmethylsulfonyl Fluoride - pharmacology PMSF, phenylmethylsulfonyl fluoride Protease Inhibitors Serine Endopeptidases Symporters TDG,β-D-galactosyl-1-thio-β-D-galactopyranoside Time Factors TLCK, N-α-p-tosyl-L-lysine chloromethyl ketone TMG, methyl-1-thio-β-D-galactoside Tosyllysine Chloromethyl Ketone - pharmacology TPCK, L-1-tosylamide-2-phenylethyl chloromethyl ketone α-NPG, p-nitrophenyl-α-D-galactoside
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Zusammenfassung:	The lactose permease of E. coli becomes irreversibly inactivated during lactose transport under conditions of high respiratory activity. This inactivation is characterized by a decrease in the steady state of lactose accumulation, a decrease in the influx rate of lactose, and a decrease in the transmembrane electrical potential. We report here that inhibitors of serine proteases (phenylmethylsulfonyl fluoride and N-α- P-tosyl-L-lysine chloromethyl ketone) prevent this inactivation, thus implicating proteases in this process.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)81209-6