Histoimmunological identification of the prolactin-like substance in rodent testis

Anti-rat prolactin (PRL) antibodies were localized by histoimmunological methods in the cytoplasm of testicular interstitial cells, Sertoli cells, spermatogonia and primary spermatocytes of the rat and mouse. Control of specificity by affinity chromatography methods showed this PRL-like material to...

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Veröffentlicht in:	Cell and tissue research 1985-01, Vol.240 (3), p.663-667
Hauptverfasser:	ROUX, M, MARTINAT, N, RICHOUX, J.-P, GRIGNON, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology Histocytochemistry Hormones and neuropeptides. Regulation Hypothalamus. Hypophysis. Epiphysis. Urophysis Immunoenzyme Techniques Leydig Cells - analysis Male Mice Mice, Inbred Strains Pituitary Gland, Anterior - analysis Prolactin - analysis Rats Rats, Inbred Strains Seminiferous Tubules - analysis Sertoli Cells - analysis Spermatozoa - analysis Testis - analysis Vertebrates: endocrinology
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Beschreibung
Zusammenfassung:	Anti-rat prolactin (PRL) antibodies were localized by histoimmunological methods in the cytoplasm of testicular interstitial cells, Sertoli cells, spermatogonia and primary spermatocytes of the rat and mouse. Control of specificity by affinity chromatography methods showed this PRL-like material to be non-specific in these testicular tissues, but specific in adenohypophyseal cells. These results are discussed.
ISSN:	0302-766X 1432-0878
DOI:	10.1007/bf00216354