Assay of β-glucuronidase in bile following ion-pair extraction of pigments and bile acids

A method for improving the assay of β-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-l-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of β-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.