Kinetoplast DNA from Trypanosoma vivax and T. congolense

We have analysed kinetoplast DNA (kDNA) of the African trypanosomes Trypanosoma vivax and T. congolense. The maxi-circles from these organisms resemble those of T. brucei in size, but only to a limited extent in sequence as judged from restriction enzyme digests and DNA-DNA hybridization. The kDNA n...

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Veröffentlicht in:	Molecular and biochemical parasitology 1985-05, Vol.15 (2), p.129-142
Hauptverfasser:	Borst, Piet, Fase-Fowler, Francesca, Weijers, Peter J., Barry, J.Dave, Tetley, Laurence, Vickerman, Keith
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences DNA, Circular - analysis DNA, Kinetoplast Electrophoresis, Agar Gel Fundamental and applied biological sciences. Psychology Human protozoal diseases Infectious diseases Kinetoplast DNA Maxi-circle Medical sciences Mini-circle Parasitic diseases Protozoa Protozoal diseases Tropical medicine Trypanosoma - analysis Trypanosoma - ultrastructure Trypanosoma congolense Trypanosoma congolense - analysis Trypanosoma congolense - ultrastructure Trypanosoma vivax Trypanosomiasis
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Zusammenfassung:	We have analysed kinetoplast DNA (kDNA) of the African trypanosomes Trypanosoma vivax and T. congolense. The maxi-circles from these organisms resemble those of T. brucei in size, but only to a limited extent in sequence as judged from restriction enzyme digests and DNA-DNA hybridization. The kDNA networks of T. vivax have three distinguishing features: they contain the highest maxi-circle concentration of any kDNA (at least twice that of T. brucei); they contain the smallest mini-circles (465 bp) yet found thusfar and the width of the kDNA nucleoid in thin sections is correspondingly small (55 nm against 91 nm for T. brucei); they contain a substantial fraction of mini-circle dimers.
ISSN:	0166-6851 1872-9428
DOI:	10.1016/0166-6851(85)90114-8