Inhibition of [3H]thymidine incorporation by Mycoplasma arginini‐infected cells due to enzymatic cleavage of the nucleoside
Culture supernatants contaminated by Mycoplasma arginini inhibit the incorporation of [3H]thymidine ([3H]dThd) by cytotoxic T lymphocyte cell lines. This study presents evidence that the inhibition of uptake of the nucleoside is due to the rapid cleavage of the exogenous [3H]dThd into thymine. Undin...
Gespeichert in:
Veröffentlicht in: | European journal of immunology 1985, Vol.15 (7), p.692-696 |
---|---|
Hauptverfasser: | , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Culture supernatants contaminated by Mycoplasma arginini inhibit the incorporation of [3H]thymidine ([3H]dThd) by cytotoxic T lymphocyte cell lines. This study presents evidence that the inhibition of uptake of the nucleoside is due to the rapid cleavage of the exogenous [3H]dThd into thymine. Undine and cytidine as well as dThd are degraded by the mycoplasma‐contaminated supernatants, while no cleavage was observed with uninfected supernatants. Cells contaminated by mycoplasma apparently release a pyrimidine‐specific nucleosidase, possibly a dThd phosphorylase, which is responsible for the inhibition. |
---|---|
ISSN: | 0014-2980 1521-4141 |
DOI: | 10.1002/eji.1830150710 |