Decarboxylation of l-Dopa by cultured mouse astrocytes
Cultured astrocytes contain immunologically specific aromatic l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis....
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| Veröffentlicht in: | Brain research 1993-10, Vol.626 (1), p.306-309 |
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| container_title | Brain research |
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| creator | Juorio, Augusto V. Li, Xin-Min Walz, Wolfgang Paterson, I. Alick |
| description | Cultured astrocytes contain immunologically specific aromatic
l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with
l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis. The formation of dopamine was blocked by NSD-1015. These experiments show that mouse cultured astrocytes are capable to convert
l-Dopa into dopamine in a dose-dependent fashion. |
| doi_str_mv | 10.1016/0006-8993(93)90592-B |
| format | Article |
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l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with
l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis. The formation of dopamine was blocked by NSD-1015. These experiments show that mouse cultured astrocytes are capable to convert
l-Dopa into dopamine in a dose-dependent fashion.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(93)90592-B</identifier><identifier>PMID: 8281440</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Aromatic l-amino acid decarboxylase ; Aromatic-L-Amino-Acid Decarboxylases - analysis ; Aromatic-L-Amino-Acid Decarboxylases - genetics ; Astrocyte ; Astrocytes - enzymology ; Astrocytes - metabolism ; Biological and medical sciences ; Cells, Cultured ; Decarboxylation ; Dopamine ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Isolated neuron and nerve. Neuroglia ; Levodopa - metabolism ; Mice ; NSD-1015 ; Pyridoxal 5-phosphate ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1993-10, Vol.626 (1), p.306-309</ispartof><rights>1993 Elsevier Science Publishers B.V. All rights reserved</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/000689939390592B$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3762550$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8281440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Juorio, Augusto V.</creatorcontrib><creatorcontrib>Li, Xin-Min</creatorcontrib><creatorcontrib>Walz, Wolfgang</creatorcontrib><creatorcontrib>Paterson, I. Alick</creatorcontrib><title>Decarboxylation of l-Dopa by cultured mouse astrocytes</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Cultured astrocytes contain immunologically specific aromatic
l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with
l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis. The formation of dopamine was blocked by NSD-1015. These experiments show that mouse cultured astrocytes are capable to convert
l-Dopa into dopamine in a dose-dependent fashion.</description><subject>Animals</subject><subject>Aromatic l-amino acid decarboxylase</subject><subject>Aromatic-L-Amino-Acid Decarboxylases - analysis</subject><subject>Aromatic-L-Amino-Acid Decarboxylases - genetics</subject><subject>Astrocyte</subject><subject>Astrocytes - enzymology</subject><subject>Astrocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Decarboxylation</subject><subject>Dopamine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Levodopa - metabolism</subject><subject>Mice</subject><subject>NSD-1015</subject><subject>Pyridoxal 5-phosphate</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1Lw0AQhhdRaq3-A4UcRPQQ3c1mJ9mLYFu_oOBFz8vuZAKRpKm7idh_b4KlV2FgmHkfhpl5GTsX_FZwAXecc4hzreW1ljeaK53E8wM2FXmWxJCk_JBN98gxOwnhcyil1HzCJnmSizTlUwZLQutd-7OtbVe166gtozpethsbuW2Efd31noqoaftAkQ2db3HbUThlR6WtA53t8ox9PD2-L17i1dvz6-JhFVMqoIs1WgcuyyUHlXIgx4kwywtHBXCLpdDAh6bDEhViaSFxBTnFrdVJgQByxq7-5m58-9VT6ExTBaS6tmsaVjIZCAUiSf8FBeSpkmoEL3Zg7xoqzMZXjfVbs_vIoF_udBvQ1qW3a6zCHpMZJEqN2P0fRsP13xV5E7CiNVJRecLOFG1lBDejUWZ0wYwumDFGo8xc_gJM6oSG</recordid><startdate>19931029</startdate><enddate>19931029</enddate><creator>Juorio, Augusto V.</creator><creator>Li, Xin-Min</creator><creator>Walz, Wolfgang</creator><creator>Paterson, I. Alick</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19931029</creationdate><title>Decarboxylation of l-Dopa by cultured mouse astrocytes</title><author>Juorio, Augusto V. ; Li, Xin-Min ; Walz, Wolfgang ; Paterson, I. Alick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e416t-9cab6b783065406eb0eec78dbed60acf1960eb0bcfc5ccfa62bdeb50aa92dc663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Aromatic l-amino acid decarboxylase</topic><topic>Aromatic-L-Amino-Acid Decarboxylases - analysis</topic><topic>Aromatic-L-Amino-Acid Decarboxylases - genetics</topic><topic>Astrocyte</topic><topic>Astrocytes - enzymology</topic><topic>Astrocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Decarboxylation</topic><topic>Dopamine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Levodopa - metabolism</topic><topic>Mice</topic><topic>NSD-1015</topic><topic>Pyridoxal 5-phosphate</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Juorio, Augusto V.</creatorcontrib><creatorcontrib>Li, Xin-Min</creatorcontrib><creatorcontrib>Walz, Wolfgang</creatorcontrib><creatorcontrib>Paterson, I. Alick</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Juorio, Augusto V.</au><au>Li, Xin-Min</au><au>Walz, Wolfgang</au><au>Paterson, I. Alick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decarboxylation of l-Dopa by cultured mouse astrocytes</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1993-10-29</date><risdate>1993</risdate><volume>626</volume><issue>1</issue><spage>306</spage><epage>309</epage><pages>306-309</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Cultured astrocytes contain immunologically specific aromatic
l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with
l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis. The formation of dopamine was blocked by NSD-1015. These experiments show that mouse cultured astrocytes are capable to convert
l-Dopa into dopamine in a dose-dependent fashion.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8281440</pmid><doi>10.1016/0006-8993(93)90592-B</doi><tpages>4</tpages></addata></record> |
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| ispartof | Brain research, 1993-10, Vol.626 (1), p.306-309 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Aromatic l-amino acid decarboxylase Aromatic-L-Amino-Acid Decarboxylases - analysis Aromatic-L-Amino-Acid Decarboxylases - genetics Astrocyte Astrocytes - enzymology Astrocytes - metabolism Biological and medical sciences Cells, Cultured Decarboxylation Dopamine Fundamental and applied biological sciences. Psychology Gene Expression Isolated neuron and nerve. Neuroglia Levodopa - metabolism Mice NSD-1015 Pyridoxal 5-phosphate Vertebrates: nervous system and sense organs |
| title | Decarboxylation of l-Dopa by cultured mouse astrocytes |
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