Decarboxylation of l-Dopa by cultured mouse astrocytes

Cultured astrocytes contain immunologically specific aromatic l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis....

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Veröffentlicht in:	Brain research 1993-10, Vol.626 (1), p.306-309
Hauptverfasser:	Juorio, Augusto V., Li, Xin-Min, Walz, Wolfgang, Paterson, I. Alick
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aromatic l-amino acid decarboxylase Aromatic-L-Amino-Acid Decarboxylases - analysis Aromatic-L-Amino-Acid Decarboxylases - genetics Astrocyte Astrocytes - enzymology Astrocytes - metabolism Biological and medical sciences Cells, Cultured Decarboxylation Dopamine Fundamental and applied biological sciences. Psychology Gene Expression Isolated neuron and nerve. Neuroglia Levodopa - metabolism Mice NSD-1015 Pyridoxal 5-phosphate Vertebrates: nervous system and sense organs
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Zusammenfassung:	Cultured astrocytes contain immunologically specific aromatic l-amino acid decarboxylase (AADC) protein and express the AADC gene. Following incubation with l-Dopa, glial cultures synthesize and metabolize dopamine. The addition of pyridoxal 5-phosphate did not change the rate of dopamine synthesis. The formation of dopamine was blocked by NSD-1015. These experiments show that mouse cultured astrocytes are capable to convert l-Dopa into dopamine in a dose-dependent fashion.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/0006-8993(93)90592-B