Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor
Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1993-12, Vol.197 (3), p.1530-1535 |
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| creator | Gunji, K. Endo, T. Ohno, M. Minegishi, T. Onaya, T. |
| description | Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO-rTSH-R cells dose-dependently, and the maximum response was obtained at 1 mIU/ml. hCG also increased cAMP in the cells from 104 mIU/ml, and the maximum stimulation was observed at 106 mIU/ml. 107 mIU of hCG had the same potency with 1 mIU of TSH to rTSH-R. On the other hand, 10−1 mIU/ml of hCG increased cAMP content in CHO-rCG-R cells and reached the maximum level at 102 mIU/ml. TSH also showed the stimulatory activity to CHO-rCG-R cells. It increased cAMP formation in CHO-rCG-R cells dose-dependently from 10 mIU/ml to 103 mIU/ml. Thirty mIU of TSH was calculated to be equivalent to 1 mIU of hCG to CG-R. These results indicate that TSH and hCG cross-react each other with rTSH-R or rCG-R. |
| doi_str_mv | 10.1006/bbrc.1993.2651 |
| format | Article |
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TSH stimulated cAMP formation in CHO-rTSH-R cells dose-dependently, and the maximum response was obtained at 1 mIU/ml. hCG also increased cAMP in the cells from 104 mIU/ml, and the maximum stimulation was observed at 106 mIU/ml. 107 mIU of hCG had the same potency with 1 mIU of TSH to rTSH-R. On the other hand, 10−1 mIU/ml of hCG increased cAMP content in CHO-rCG-R cells and reached the maximum level at 102 mIU/ml. TSH also showed the stimulatory activity to CHO-rCG-R cells. It increased cAMP formation in CHO-rCG-R cells dose-dependently from 10 mIU/ml to 103 mIU/ml. Thirty mIU of TSH was calculated to be equivalent to 1 mIU of hCG to CG-R. These results indicate that TSH and hCG cross-react each other with rTSH-R or rCG-R.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1993.2651</identifier><identifier>PMID: 8280172</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; CHO Cells ; Chorionic Gonadotropin - pharmacology ; Clone Cells ; Cricetinae ; Cyclic AMP - metabolism ; Fundamental and applied biological sciences. Psychology ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; Humans ; Kinetics ; Molecular and cellular biology ; Rats ; Receptors, LH - biosynthesis ; Receptors, LH - metabolism ; Receptors, Thyrotropin - biosynthesis ; Receptors, Thyrotropin - metabolism ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - metabolism ; Recombinant Proteins - pharmacology ; Thyrotropin - pharmacology ; Transfection</subject><ispartof>Biochemical and biophysical research communications, 1993-12, Vol.197 (3), p.1530-1535</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-64a83dbdb59cbc72804a65090b9f2a58f0db168ebade639c8ef8b3e5103faf733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1993.2651$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3883717$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8280172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gunji, K.</creatorcontrib><creatorcontrib>Endo, T.</creatorcontrib><creatorcontrib>Ohno, M.</creatorcontrib><creatorcontrib>Minegishi, T.</creatorcontrib><creatorcontrib>Onaya, T.</creatorcontrib><title>Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO-rTSH-R cells dose-dependently, and the maximum response was obtained at 1 mIU/ml. hCG also increased cAMP in the cells from 104 mIU/ml, and the maximum stimulation was observed at 106 mIU/ml. 107 mIU of hCG had the same potency with 1 mIU of TSH to rTSH-R. On the other hand, 10−1 mIU/ml of hCG increased cAMP content in CHO-rCG-R cells and reached the maximum level at 102 mIU/ml. TSH also showed the stimulatory activity to CHO-rCG-R cells. It increased cAMP formation in CHO-rCG-R cells dose-dependently from 10 mIU/ml to 103 mIU/ml. Thirty mIU of TSH was calculated to be equivalent to 1 mIU of hCG to CG-R. These results indicate that TSH and hCG cross-react each other with rTSH-R or rCG-R.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>CHO Cells</subject><subject>Chorionic Gonadotropin - pharmacology</subject><subject>Clone Cells</subject><subject>Cricetinae</subject><subject>Cyclic AMP - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Rats</subject><subject>Receptors, LH - biosynthesis</subject><subject>Receptors, LH - metabolism</subject><subject>Receptors, Thyrotropin - biosynthesis</subject><subject>Receptors, Thyrotropin - metabolism</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Thyrotropin - pharmacology</subject><subject>Transfection</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFP2zAYhq1piHXdrrsh-TDtls6OG8c5VlELCCYQMGk3y3a-gKfEDrY7wb-fqxbEhZMP7_O9ev0g9I2SBSWE_9Q6mAVtGrYoeUU_oBklDSlKSpYf0Yxkoigb-ucT-hzjX0IoXfLmGB2LUhBalzP0dAPGj9o65RK-e3gOPgU_WYdvkx23g0oQsVn9usYbH0aVrHc4h-3ZVXFBcQvDEPH6aQoQo3X3-G1Z--BDxq3Bp96p7qU3IzAlH76go14NEb4e3jn6vVnftWfF5dXpebu6LAzjIhV8qQTrdKerxmhT59lLxav8Rd30papETzpNuQCtOuCsMQJ6oRlUlLBe9TVjc_Rj3zsF_7iFmORoo8nDlQO_jbLmtGKkJhlc7EETfIwBejkFO6rwLCmRO9Vyp1ruVMud6nxwcmje6hG6V_zgNuffD7mKRg19UM7Y-IoxIVhN64yJPQbZwj8LQUZjwRnobACTZOftewv-A7JBnCU</recordid><startdate>19931230</startdate><enddate>19931230</enddate><creator>Gunji, K.</creator><creator>Endo, T.</creator><creator>Ohno, M.</creator><creator>Minegishi, T.</creator><creator>Onaya, T.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931230</creationdate><title>Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor</title><author>Gunji, K. ; Endo, T. ; Ohno, M. ; Minegishi, T. ; Onaya, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-64a83dbdb59cbc72804a65090b9f2a58f0db168ebade639c8ef8b3e5103faf733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>CHO Cells</topic><topic>Chorionic Gonadotropin - pharmacology</topic><topic>Clone Cells</topic><topic>Cricetinae</topic><topic>Cyclic AMP - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Rats</topic><topic>Receptors, LH - biosynthesis</topic><topic>Receptors, LH - metabolism</topic><topic>Receptors, Thyrotropin - biosynthesis</topic><topic>Receptors, Thyrotropin - metabolism</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - metabolism</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Thyrotropin - pharmacology</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gunji, K.</creatorcontrib><creatorcontrib>Endo, T.</creatorcontrib><creatorcontrib>Ohno, M.</creatorcontrib><creatorcontrib>Minegishi, T.</creatorcontrib><creatorcontrib>Onaya, T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gunji, K.</au><au>Endo, T.</au><au>Ohno, M.</au><au>Minegishi, T.</au><au>Onaya, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1993-12-30</date><risdate>1993</risdate><volume>197</volume><issue>3</issue><spage>1530</spage><epage>1535</epage><pages>1530-1535</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO-rTSH-R cells dose-dependently, and the maximum response was obtained at 1 mIU/ml. hCG also increased cAMP in the cells from 104 mIU/ml, and the maximum stimulation was observed at 106 mIU/ml. 107 mIU of hCG had the same potency with 1 mIU of TSH to rTSH-R. On the other hand, 10−1 mIU/ml of hCG increased cAMP content in CHO-rCG-R cells and reached the maximum level at 102 mIU/ml. TSH also showed the stimulatory activity to CHO-rCG-R cells. It increased cAMP formation in CHO-rCG-R cells dose-dependently from 10 mIU/ml to 103 mIU/ml. Thirty mIU of TSH was calculated to be equivalent to 1 mIU of hCG to CG-R. These results indicate that TSH and hCG cross-react each other with rTSH-R or rCG-R.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8280172</pmid><doi>10.1006/bbrc.1993.2651</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1993-12, Vol.197 (3), p.1530-1535 |
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| subjects | Animals Biological and medical sciences Cell receptors Cell structures and functions CHO Cells Chorionic Gonadotropin - pharmacology Clone Cells Cricetinae Cyclic AMP - metabolism Fundamental and applied biological sciences. Psychology Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors Humans Kinetics Molecular and cellular biology Rats Receptors, LH - biosynthesis Receptors, LH - metabolism Receptors, Thyrotropin - biosynthesis Receptors, Thyrotropin - metabolism Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Thyrotropin - pharmacology Transfection |
| title | Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor |
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