Recombinant Thyrotropin Stimulates cAMP Formation in CHO-K1 Cells Expressing Recombinant Chorionic Gonadotropin Receptor

Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO...

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Veröffentlicht in:Biochemical and biophysical research communications 1993-12, Vol.197 (3), p.1530-1535
Hauptverfasser: Gunji, K., Endo, T., Ohno, M., Minegishi, T., Onaya, T.
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Sprache:eng
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Zusammenfassung:Using CHO-K1 cells expressing rat thyrotropin-receptor (CHO-rTSH-R cells) or rat chorionic gonadotropin-receptor (CHO-rCG-R cells), we have examined their reactivity or cross-reactivity to recombinant human thyrotropin (TSH) or human chorionic gonadotropin (hCG). TSH stimulated cAMP formation in CHO-rTSH-R cells dose-dependently, and the maximum response was obtained at 1 mIU/ml. hCG also increased cAMP in the cells from 104 mIU/ml, and the maximum stimulation was observed at 106 mIU/ml. 107 mIU of hCG had the same potency with 1 mIU of TSH to rTSH-R. On the other hand, 10−1 mIU/ml of hCG increased cAMP content in CHO-rCG-R cells and reached the maximum level at 102 mIU/ml. TSH also showed the stimulatory activity to CHO-rCG-R cells. It increased cAMP formation in CHO-rCG-R cells dose-dependently from 10 mIU/ml to 103 mIU/ml. Thirty mIU of TSH was calculated to be equivalent to 1 mIU of hCG to CG-R. These results indicate that TSH and hCG cross-react each other with rTSH-R or rCG-R.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.2651