Herpesvirus ICP18.5 and DNA-binding protein genes are conserved in equine herpesvirus-1

The genome of equine herpesvirus-1 (EHV-1) contained three open reading frames (ORFs) in a 3.9 kbp BamHI-SmaI fragment at 0.38-0.41 map units in the long unique region. The most 5' ORF encoded the carboxy terminus of a protein with 45-55 percent amino acid homology to the DNA-binding proteins (ICP8-DBP) of four other alpha-herpesviruses. The middle ORF translated to a polypeptide of 775 residues with 43-55% homology to the ICP18.5 proteins. The most 3' ORF encoded the EHV-1 glycoprotein B (gB) gene. Three mRNAs of 4.3, 4.4-4.8, and 3.5-3.9 kb (corresponding to the three sequenced ORFs) were all transcribed from the same strand. The gene order of this group was conserved in all herpesviruses examined.