Organ culture of adult cockroach CNS: Ultrastructural and physiological characteristics

The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected a...

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Veröffentlicht in:	Tissue & cell 1985, Vol.17 (1), p.141-145
Hauptverfasser:	Howes, E.A., Leech, C.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Action Potentials - drug effects Animals Axons - physiology Axons - ultrastructure Biological and medical sciences Blattidae blood-brain barrier Central Nervous System - physiology Central Nervous System - ultrastructure CNS Cockroach Cockroaches - anatomy & histology culture Fundamental and applied biological sciences. Psychology Ganglia - ultrastructure Insecta Intercellular Junctions - ultrastructure Invertebrates Male Microscopy, Electron Morphology. Histology. Cytology Neuroglia - ultrastructure Organ Culture Techniques Periplaneta americana Potassium - pharmacology Sodium - pharmacology
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Zusammenfassung:	The abdominal nerve cord of adult Periplaneta americana has been maintained in culture for up to 5 weeks. The ultrastructural appearance of in vitro cords resembles that of in vivo cords although some degeneration of glia may occur during the first 2 weeks in culture. The glial cells most affected are those which make up the perineurium. The blood-brain barrier, formed by the perineurium in vivo, breaks down in vitro. Despite this, normal action potentials were recorded from giant axons in 1 month old cultured connectives.
ISSN:	0040-8166 1532-3072
DOI:	10.1016/0040-8166(85)90022-9