Hemagglutination assay for human serum fibronectin

A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentra...

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Veröffentlicht in:	Journal of immunological methods 1985-05, Vol.79 (1), p.65-70
Hauptverfasser:	Bǎlunǎ, Roxana-Georgeta, Barac, Doina, Tarnavschi, Ruxandra, Belaşcu, Irena
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Epitopes Erythrocytes - immunology Exact sciences and technology fibronectin Fibronectins - blood Fibronectins - metabolism gelatin Gelatin - metabolism hemagglutination Hemagglutination Tests - methods Humans Other techniques and industries Sheep - immunology
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Beschreibung
Zusammenfassung:	A hemagglutination assay for human serum fibronectin (Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentration giving positive hemagglutination was found to be 7 μg/ml, a sensitivity making it suitable for measurement of Fn in both normal and pathologic sera. The assay is simple and rapid and does not require specialized equipment.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(85)90392-8