Effects of postmortem injection time, injection level, and concentration of calcium chloride on beef quality traits

Two experiments were conducted to determine the optimum protocol for maximizing meat quality with CaCl2 injection. Experiment 1 compared the effects of 30 min or 24 h postmortem injection of 175 mM CaCl2 or water at 10% (wt/wt) to controls on various measures of meat quality. An injection of CaCl2 reduced (P .05) shear force values in all three beef muscles evaluated (longissimus, semimembranosus, and triceps brachii). Retail lean color was not affected (P 0.05) by a CaCl2 injection at 24 h postmortem, but was slightly darker (P 0.05) for an injection at 30 min postmortem. Psychrophilic and total aerobic microbial counts were higher (P 0.0 5) in 30-min than in 24-h treatments and were higher (P 0.05) in CaCl2- or water-injected semimembranosus muscle than in the control, but the treatment differences were minimal in 24-h injected meat. Experiment 2 compared the effects of 24-h postmortem injection of 200 mM or 250 mM CaCl2 at either 5 or 10% (wt/wt) to controls on meat quality traits. Both 200 and 250 mM CaCl2 reduced (P 0.05) shear force values compared with the control. In addition, variation in shear force was decreased (P 0.05) with CaCl2 injection. Beef flavor intensity was slightly lower (P 0.05) and off-flavor ratings were slightly higher (P 0.05) in CaCl2-injected meat, but the small differences were of no practical significance. Retail lean color was not affected (P .05) by injection of the CaCl2 at 24 h. Injecting 200 mM CaCl2 at 5% (wt/wt) into 24 h postmortem meat can produce consistently tender meat without compromising other palatability or lean quality traits