Bluetongue virus: production and study of viral antigen for serological diagnosis

A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 10 3 and 25 × 10 3 NMWP, showed complete identity to standard antigens when compared by agar gel...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of virological methods 1993-10, Vol.44 (2), p.281-286
Hauptverfasser:	Arita, G.M.M., Gatti, M.S.V., Deak, J.G., De Castro, A.F.Pestana
Format:	Artikel
Sprache:	eng
Schlagworte:	Agar gel immunodiffusion Animals Antibodies, Viral - blood Antigen-Antibody Reactions ANTIGENE ANTIGENOS ANTIGENS Antigens, Viral - chemistry Antigens, Viral - immunology Biological and medical sciences Bluetongue - diagnosis bluetongue virus Bluetongue virus - chemistry Bluetongue virus - growth & development Bluetongue virus - immunology Bluetongue virus serotype 4 Electrophoresis, Polyacrylamide Gel Epitopes Fundamental and applied biological sciences. Psychology IMMUNODIAGNOSIS IMMUNODIAGNOSTIC Immunodiffusion INMUNODIAGNOSTICO Microbiology NS 1 protein ORBIVIRUS SEROTIPOS SEROTYPE SEROTYPES Sheep Soluble antigen Techniques used in virology Vero Cells Virology Virus Cultivation
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A soluble antigen, produced from the culture supernatant of VERO cells infected with bluetongue virus serotype 4 (BTV-S4) and concentrated by sequential ultrafiltration with membranes with cut-off values 10 3 and 25 × 10 3 NMWP, showed complete identity to standard antigens when compared by agar gel immunodiffusion (AGID) and SDS-PAGE profiles, revealing that the main protein component responsible for the AGID reaction has a molecular weight of about 60 kDa corresponding probably to the NS 1 protein.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(93)90063-W