An improved fluorimetric assay for brain monoamine oxidase

A fluorimetric assay for monamine oxidase that uses kynuramine as the substrate is described. This method is more economic, rapid, reproducible, and sensitive than other similar procedures. Its validity has been confirmed by the study of the subcellular distribution, the effects of inhibitors, and t...

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Veröffentlicht in:	Journal of pharmacological methods 1985-01, Vol.13 (3), p.213-223
Hauptverfasser:	Morinan, Alun, Garratt, Helen M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Brain - enzymology Clorgyline Clorgyline - pharmacology Deprenyl Enzymes and enzyme inhibitors Fluorescence Fundamental and applied biological sciences. Psychology Hydroxyquinolines - metabolism Kinetics Kynuramine Kynuramine - pharmacology Male Monoamine oxidase Monoamine Oxidase - analysis Monoamine Oxidase Inhibitors - pharmacology Neurochemistry Oxidoreductases Rats Rats, Inbred Strains Selegiline - pharmacology Spectrometry, Fluorescence
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Beschreibung
Zusammenfassung:	A fluorimetric assay for monamine oxidase that uses kynuramine as the substrate is described. This method is more economic, rapid, reproducible, and sensitive than other similar procedures. Its validity has been confirmed by the study of the subcellular distribution, the effects of inhibitors, and the kinetics of enzyme activity. Previous reports of substrate inhibition by kynuramine may have to be reassessed in the light of evidence presented here.
ISSN:	0160-5402
DOI:	10.1016/0160-5402(85)90021-X