A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma
Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. In this study, a modification of the immunoperoxidase method was shown to enable the detection o...
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Veröffentlicht in: | Experimental pathology (1981) 1985, Vol.27 (2), p.119-122 |
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container_issue | 2 |
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container_title | Experimental pathology (1981) |
container_volume | 27 |
creator | Stul, M.S. Logghe, G.N. Bergmans, G.B. Vanvuchelen, J.K. |
description | Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. In this study, a modification of the immunoperoxidase method was shown to enable the detection of BGAg in deparaffinized tissue section of normal and malignant human urothelium. To detect antigens A and B a peroxidase-conjugated rabbit anti-human IgM was used and antigen H was visualized by biotinylated lectin. |
doi_str_mv | 10.1016/S0232-1513(85)80049-9 |
format | Article |
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In this study, a modification of the immunoperoxidase method was shown to enable the detection of BGAg in deparaffinized tissue section of normal and malignant human urothelium. To detect antigens A and B a peroxidase-conjugated rabbit anti-human IgM was used and antigen H was visualized by biotinylated lectin.</description><identifier>ISSN: 0232-1513</identifier><identifier>DOI: 10.1016/S0232-1513(85)80049-9</identifier><identifier>PMID: 3888650</identifier><language>eng</language><publisher>Germany</publisher><subject>ABH tissue isoantigens ; ABO Blood-Group System - immunology ; carcinoma ; Carcinoma, Transitional Cell - immunology ; human ; Humans ; Immunoenzyme Techniques ; immunoperoxidase method ; Isoantigens - analysis ; urinary bladder ; Urinary Bladder - immunology ; Urinary Bladder Neoplasms - immunology ; urothelium</subject><ispartof>Experimental pathology (1981), 1985, Vol.27 (2), p.119-122</ispartof><rights>1985</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c308t-a16277ce0a80a309683e3160c2a24567edd6b42cfae4274ba0e6e28a07f003eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3888650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stul, M.S.</creatorcontrib><creatorcontrib>Logghe, G.N.</creatorcontrib><creatorcontrib>Bergmans, G.B.</creatorcontrib><creatorcontrib>Vanvuchelen, J.K.</creatorcontrib><title>A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma</title><title>Experimental pathology (1981)</title><addtitle>Exp Pathol</addtitle><description>Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. In this study, a modification of the immunoperoxidase method was shown to enable the detection of BGAg in deparaffinized tissue section of normal and malignant human urothelium. To detect antigens A and B a peroxidase-conjugated rabbit anti-human IgM was used and antigen H was visualized by biotinylated lectin.</description><subject>ABH tissue isoantigens</subject><subject>ABO Blood-Group System - immunology</subject><subject>carcinoma</subject><subject>Carcinoma, Transitional Cell - immunology</subject><subject>human</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>immunoperoxidase method</subject><subject>Isoantigens - analysis</subject><subject>urinary bladder</subject><subject>Urinary Bladder - immunology</subject><subject>Urinary Bladder Neoplasms - immunology</subject><subject>urothelium</subject><issn>0232-1513</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFP3DAQhX1oBQj4CUg-Ve0hZWwnjnOqtogCEhKHlrPl2JPuVJt4sZ0C_57Arrgyl5HmvTej-Rg7E_BdgNDnv0EqWYlGqK-m-WYA6q7qPrGj9_EhO835HyylpTbCHLADZYzRDRyxhxUfY6CBMHAax3mKW0zxiYLLyEcs6xj4EBMva-QBC_pCceJx4Kuf17xQzjNyytFNhf7ilDlNfOsK4VQyf6Sy5v3GhYCJe5c8TXF0J-zz4DYZT_f9mN3_uvxzcV3d3l3dXKxuK6_AlMoJLdvWIzgDTkGnjUIlNHjpZN3oFkPQfS394LCWbd07QI3SOGgHAIW9OmZfdnu3KT7MmIsdKXvcbNyEcc621ULKTjaLsdkZfYo5JxzsNtHo0rMVYF8J2zfC9hWlNY19I2y7JXe2PzD3I4b31J7tov_Y6bh8-Z8w2ewXMB4DpYWjDZE-uPACMM-OFA</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Stul, M.S.</creator><creator>Logghe, G.N.</creator><creator>Bergmans, G.B.</creator><creator>Vanvuchelen, J.K.</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma</title><author>Stul, M.S. ; Logghe, G.N. ; Bergmans, G.B. ; Vanvuchelen, J.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-a16277ce0a80a309683e3160c2a24567edd6b42cfae4274ba0e6e28a07f003eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>ABH tissue isoantigens</topic><topic>ABO Blood-Group System - immunology</topic><topic>carcinoma</topic><topic>Carcinoma, Transitional Cell - immunology</topic><topic>human</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>immunoperoxidase method</topic><topic>Isoantigens - analysis</topic><topic>urinary bladder</topic><topic>Urinary Bladder - immunology</topic><topic>Urinary Bladder Neoplasms - immunology</topic><topic>urothelium</topic><toplevel>online_resources</toplevel><creatorcontrib>Stul, M.S.</creatorcontrib><creatorcontrib>Logghe, G.N.</creatorcontrib><creatorcontrib>Bergmans, G.B.</creatorcontrib><creatorcontrib>Vanvuchelen, J.K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental pathology (1981)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stul, M.S.</au><au>Logghe, G.N.</au><au>Bergmans, G.B.</au><au>Vanvuchelen, J.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma</atitle><jtitle>Experimental pathology (1981)</jtitle><addtitle>Exp Pathol</addtitle><date>1985</date><risdate>1985</risdate><volume>27</volume><issue>2</issue><spage>119</spage><epage>122</epage><pages>119-122</pages><issn>0232-1513</issn><abstract>Detectable ABH blood group antigens (BGAg) on tumor cells from transitional carcinoma of the urinary bladder, used for diagnosis and prognosis may be evidenced with the existing immunoperoxidase methods. 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subjects | ABH tissue isoantigens ABO Blood-Group System - immunology carcinoma Carcinoma, Transitional Cell - immunology human Humans Immunoenzyme Techniques immunoperoxidase method Isoantigens - analysis urinary bladder Urinary Bladder - immunology Urinary Bladder Neoplasms - immunology urothelium |
title | A modified immunoperoxidase method for the detection of ABH tissue isoantigens in patients with bladder carcinoma |
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