Dexamethasone Sensitizes Soluble Guanylate Cyclase in the Rat Renal Glomeruli

Dexamethasone Sensitizes Soluble Guanylate Cyclase in the Rat Renal Glomeruli

We measured capability for guanosine 3′,5′-cyclic monophosphate (cGMP) synthesis by isolated renal glomeruli from steroid-treated rats. Glomeruli obtained from animals treated with dexamethasone showed 5-fold higher sensitivity to sodium nitroprusside (SNP) than glomeruli from deoxycorticosterone- o...

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Veröffentlicht in:Biochemical and biophysical research communications 1993-12, Vol.197 (2), p.826-832
Hauptverfasser: Lewko, B., Wendt, U., Stepinski, J., Angielski, S.
Format: Artikel
Sprache:eng
Schlagworte:
1-Methyl-3-isobutylxanthine - pharmacology
Animals
Biological and medical sciences
Cyclic GMP - metabolism
Cytosol - enzymology
Desoxycorticosterone - pharmacology
Dexamethasone - pharmacology
Guanylate Cyclase - metabolism
Kidney Glomerulus - drug effects
Kidney Glomerulus - enzymology
Kidney Glomerulus - metabolism
Kinetics
L-Lactate Dehydrogenase - analysis
Male
Medical sciences
Nitroprusside - pharmacology
Pharmacology. Drug treatments
Rats
Rats, Wistar
Reference Values
Urinary system
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Zusammenfassung:We measured capability for guanosine 3′,5′-cyclic monophosphate (cGMP) synthesis by isolated renal glomeruli from steroid-treated rats. Glomeruli obtained from animals treated with dexamethasone showed 5-fold higher sensitivity to sodium nitroprusside (SNP) than glomeruli from deoxycorticosterone- or saline-treated rats. Similar increase in activity of the glomerular soluble guanylate cyclase in response to SNP was observed. Dexamethasone markedly suppressed stimulatory effect of atrial natriuretic factor (ANF) on glomerular cGMP synthesis suggesting existence of cross-regulatory system(s) generating cGMP in renal glomeruli. We presume that glucocorticoid-induced hyperfiltration can be partially caused by sensitization of soluble guanylate cyclase to the nitric oxide, a potent natural dilatory agent.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1993.2553