Changes in nuclear proteins induced by heat shock in Drosophila culture cells

Nuclear proteins of normal and heat-shocked Drosophila cells were analysed by two-dimensional electrophoresis. The computerized processing of the gels allowed us to detect 6 proteins strongly induced by the heat treatment, but which were different from the usually described heat-shock proteins. The...

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Veröffentlicht in:	FEBS letters 1985-05, Vol.184 (2), p.278-284
Hauptverfasser:	Rabilloud, T., Vincens, P., Hubert, M., Pennetier, J.L., Tarroux, P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences BSA, bovine serum albumin Cell Nucleus - physiology Cells, Cultured CHAPS, 3-(3-cholamidopropyldimethylammonio)propanesulfonate Drosophila Drosophila melanogaster - genetics Drosophilidae Fundamental and applied biological sciences. Psychology Gene Expression Regulation Heat-Shock Proteins - genetics Holoproteins Hot Temperature hsps, heat-shock proteins Isoelectric Point Molecular Weight NP-40, Nonidet P-40 Nuclear protein Heat-shock Genetic regulation Drosophila cell Nuclear proteins Nucleoproteins - physiology PMSF, phenylmethylsulfonyl fluoride Proteins
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Zusammenfassung:	Nuclear proteins of normal and heat-shocked Drosophila cells were analysed by two-dimensional electrophoresis. The computerized processing of the gels allowed us to detect 6 proteins strongly induced by the heat treatment, but which were different from the usually described heat-shock proteins. The possible role of these proteins in genetic regulation is discussed, as is the value of this type of approach for the study of other genetic regulation phenomena.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80622-0