Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione
Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen...
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| Veröffentlicht in: | Fertility and sterility 2010-11, Vol.94 (6), p.2409-2412 |
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| container_end_page | 2412 |
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| container_issue | 6 |
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| container_title | Fertility and sterility |
| container_volume | 94 |
| creator | Kim, Gil Ah, M.S Lee, Seung Tae, Ph.D Ahn, Ji Yeon, Ph.D Park, Jong Heum, Ph.D Lim, Jeong Mook, D.V.M., Ph.D |
| description | Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation. |
| doi_str_mv | 10.1016/j.fertnstert.2010.01.073 |
| format | Article |
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Obstetrics</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Obstetrics and Gynecology</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Stem cells</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2P0zAQhi0EYruFv4B8QZxSxnHspBckWMGy0koc-Lhajj1mXZK42E6h_HoctbASJy62ZD8z78w7QwhlsGHA5MvdxmHMU8rl3NRQnoFtoOUPyIoJISshBX9IVgBMVFB39QW5TGkHAJK19WNyUUPNpeR8Rb7cjPsYDmjpweveDz4faXDURcRfWOU7_aN84djHY5i8oUVxpAaHIVHtijrFn_uQ5og0B_p1mLPOdz5M-IQ8cnpI-PR8r8nnd28_Xb2vbj9c31y9vq2MgC5XVjS9axvDHUorOwuuwS2gbaSQpu27jjdMSynYljPBrTZGWKid3XaN2Roh-Jq8OOUtTXyfMWU1-rTUpycMc1KtZNDwVraF7E6kiSGliE7tox91PCoGajFV7dS9qWoxVQFTxdQS-uwsMvcj2r-Bf1wswPMzoJPRg4t6Mj7dc6WL7QKuyZsTh8WSg8eokvE4GbQ-osnKBv8_1bz6J4kZfJmNHr7hEdMuzHEqliumUq1AfVyWYNkBVsbPmq7mvwFD-bDC</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Kim, Gil Ah, M.S</creator><creator>Lee, Seung Tae, Ph.D</creator><creator>Ahn, Ji Yeon, Ph.D</creator><creator>Park, Jong Heum, Ph.D</creator><creator>Lim, Jeong Mook, D.V.M., Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione</title><author>Kim, Gil Ah, M.S ; Lee, Seung Tae, Ph.D ; Ahn, Ji Yeon, Ph.D ; Park, Jong Heum, Ph.D ; Lim, Jeong Mook, D.V.M., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-d54bf74c3fe6d68d0f4e90ed4656c7b88341a665193153dacc5d02fd984c9c553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>antioxidant</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Embryonic Stem Cells - drug effects</topic><topic>Embryonic Stem Cells - metabolism</topic><topic>Embryonic Stem Cells - physiology</topic><topic>Freezing</topic><topic>Glutathione - pharmacology</topic><topic>Gynecology. 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| ispartof | Fertility and sterility, 2010-11, Vol.94 (6), p.2409-2412 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals antioxidant Biological and medical sciences Cell Survival - drug effects Cell Survival - physiology Cells, Cultured cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Freezing Glutathione - pharmacology Gynecology. Andrology. Obstetrics Internal Medicine Medical sciences Mice Obstetrics and Gynecology reactive oxygen species Reactive Oxygen Species - metabolism Stem cells |
| title | Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione |
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