Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen...

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Veröffentlicht in:	Fertility and sterility 2010-11, Vol.94 (6), p.2409-2412
Hauptverfasser:	Kim, Gil Ah, M.S, Lee, Seung Tae, Ph.D, Ahn, Ji Yeon, Ph.D, Park, Jong Heum, Ph.D, Lim, Jeong Mook, D.V.M., Ph.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antioxidant Biological and medical sciences Cell Survival - drug effects Cell Survival - physiology Cells, Cultured cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Embryonic Stem Cells - drug effects Embryonic Stem Cells - metabolism Embryonic Stem Cells - physiology Freezing Glutathione - pharmacology Gynecology. Andrology. Obstetrics Internal Medicine Medical sciences Mice Obstetrics and Gynecology reactive oxygen species Reactive Oxygen Species - metabolism Stem cells
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Beschreibung
Zusammenfassung:	Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which was caused by a decrease in reactive oxygen species, was only induced by exposure of embryonic stem cells during cryopreservation.
ISSN:	0015-0282 1556-5653
DOI:	10.1016/j.fertnstert.2010.01.073