Sequence and characterization of mutT from Proteus vulgaris
A cloned DNA fragment containing the tryptophanase ( tna) operon of Proteus vulgaris was found to contain a gene analogous to mutT of Escherichia coli immediately distal to the tna operon. The presumptive mutT of P. vulgaris was shown to be a functional gene by complemention of a mutT mutant from E....
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| Veröffentlicht in: | Gene 1993-11, Vol.134 (1), p.99-102 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
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| Zusammenfassung: | A cloned DNA fragment containing the tryptophanase (
tna) operon of
Proteus vulgaris was found to contain a gene analogous to
mutT of
Escherichia coli immediately distal to the
tna operon. The presumptive
mutT of
P. vulgaris was shown to be a functional gene by complemention of a
mutT mutant from
E. coli. The deduced amino acid sequence of the MutT polypeptide of
P. vulgaris was 47% identical and 70% similar to MutT of
E. coli. The
mutT and
tna operons of
P. vulgaris were shown to be adjacent on the genome of this organism. These operons are located about 20 min apart in the
E. coli genome. Our findings suggest that either or both
tna and
mutT have different genomic locations in the two organisms. |
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| ISSN: | 0378-1119 1879-0038 |
| DOI: | 10.1016/0378-1119(93)90180-B |