Immunochemical recognition of oxytocin by antiserum to L-prolyl-leucyl-glycine amide

Antisera were raised in rabbits against Pro-Leu-Gly-NH 2 (PLG)-bovine thyroglobulin conjugates, Specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to Sepharose beads. It was concluded...

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Veröffentlicht in:	Neuroscience letters 1985-01, Vol.54 (2), p.283-288
Hauptverfasser:	Watkins, W.B., Peek, J.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Specificity antisera Anura Biological and medical sciences Chickens Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Goldfish Hormones and neuropeptides. Regulation Hypothalamus - metabolism Hypothalamus. Hypophysis. Epiphysis. Urophysis Immunoenzyme Techniques immunohistology MSH Release-Inhibiting Hormone - immunology oxytocin Oxytocin - immunology Oxytocin - metabolism Pro-Leu-Gly-NH 2 Rabbits - immunology Radioimmunoassay Rats specificity Vertebrates: endocrinology
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Beschreibung
Zusammenfassung:	Antisera were raised in rabbits against Pro-Leu-Gly-NH 2 (PLG)-bovine thyroglobulin conjugates, Specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to Sepharose beads. It was concluded that immunization with PLG can raise antisera that are specific to oxytocin.
ISSN:	0304-3940 1872-7972
DOI:	10.1016/S0304-3940(85)80092-6