Deuterium NMR spectroscopy of biofluids for the identification of drug metabolites: Application to N, N-dimethylformamide

The metabolism of N, N-dimethylformamide in the Sprague—Dawley rat has been reinvestigated using NMR spectroscopy of urine. In particular, through the use of N, N-dimethylformamide-d 7 (DMF-d 7) and 2H-NMR spectroscopy, the principal metabolites of this compound have been confirmed in a direct manner. The use of inverse-detected two-dimensional 2H 13C correlation with 13C decoupling aided metabolite identification through the provision of 13C chemical shifts.