Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10(8.5), 10(3.5), an...

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Veröffentlicht in:	Archives of virology 1993-01, Vol.133 (3/4), p.477-483
Hauptverfasser:	Kim, H.S, Kwang, J, Yoon, I.J, Joo, H.S, Frey, M.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Alphaarterivirus Animals Arterivirus Arterivirus - physiology Biological and medical sciences cell culture Cell Line cell lines Clone Cells Cytopathogenic Effect, Viral Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Haplorhini kidneys Microbiology monkeys replication Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA Viruses - physiology Swine swine diseases Virology Virus Replication
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Beschreibung
Zusammenfassung:	Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10(8.5), 10(3.5), and 10(2.5) tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01313785