An evaluation of commercial discs for the determination of the growth factor requirements of avian haemophili

The ability of 2 brands of growth factor discs (Oxoid and Mast) to identify correctly the growth factor requirements of 41 isolates of Haemophilus paragallinarum and 17 isolates of H. avium was evaluated. The percentage of isolates correctly identified as requiring V factor varied with both the bran...

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Veröffentlicht in:	Veterinary microbiology 1985, Vol.10 (2), p.125-131
Hauptverfasser:	Blackall, P.J., Farrah, J.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Birds - microbiology Culture Media Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Haemophilus - drug effects Haemophilus - growth & development Haemophilus paragallinarum Hemin - pharmacology Microbiology NAD - pharmacology
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Zusammenfassung:	The ability of 2 brands of growth factor discs (Oxoid and Mast) to identify correctly the growth factor requirements of 41 isolates of Haemophilus paragallinarum and 17 isolates of H. avium was evaluated. The percentage of isolates correctly identified as requiring V factor varied with both the brand of the disc and the medium used. On basic nutrient media both brands gave low percentages of correct results: on Isosensitest Agar (Oxoid) the Oxoid discs gave 26% and the Mast discs 24% while on Heart Infusion Agar (Gibco) the Oxoid discs gave 54% and the Mast discs 28%. However, on a more complex medium (TM/S) the percentage of correct results was considerably higher with the Oxoid discs 100% and the Mast discs 52% accurate.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/0378-1135(85)90014-8