An assay for GDP- d-mannose-4,6-dehydratase
The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C 5 hydrogen has been established using GDP- d-[5- 3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.
Gespeichert in:
| Veröffentlicht in: | Anal. Biochem.; (United States) 1985, Vol.144 (1), p.253-257 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 257 |
|---|---|
| container_issue | 1 |
| container_start_page | 253 |
| container_title | Anal. Biochem.; (United States) |
| container_volume | 144 |
| creator | Chang, Sulie Broschat, Kay O. Serif, George S. |
| description | The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C
5 hydrogen has been established using GDP-
d-[5-
3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of
3H with the aqueous medium. |
| doi_str_mv | 10.1016/0003-2697(85)90113-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_76071903</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0003269785901137</els_id><sourcerecordid>76071903</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-1193e8ce4ff18f3c2ba213b7d89d1335390a2a0c858bd80bdca083e48a99c413</originalsourceid><addsrcrecordid>eNqFkMFKAzEQhoMotVbfQKGIiKLRySbZTS5CqVqFgh56D9lklq60u5pshb69u7b0qKc5_N8M83-EnDK4Y8DSewDgNEl1dqXktQbGOM32SJ-BTilw0Pukv0MOyVGMH9BCQqY90kukAiFln9yMqqGN0a6HRR2Gk8d3OvR0aauqjkjFbUo9ztc-2MZGPCYHhV1EPNnOAZk9P83GL3T6Nnkdj6bUCSEaypjmqByKomCq4C7JbcJ4nnmlPeNccg02seCUVLlXkHtnQXEUymrtBOMDcr45W8emNNGVDbq5q6sKXWNkqlgGHXS5gT5D_bXC2JhlGR0uFrbCehVNlkLGNPB_QSZAZwlPW1BsQBfqGAMW5jOUSxvWhoHphJvOpulsGiXNr3CTtWtn2_urfIl-t7Q13OYX29xGZxdFsJUr4w5TQiVSQIs9bDBsxX6XGLriWDn0Zeh6-7r8-48fFymXuQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14097236</pqid></control><display><type>article</type><title>An assay for GDP- d-mannose-4,6-dehydratase</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Chang, Sulie ; Broschat, Kay O. ; Serif, George S.</creator><creatorcontrib>Chang, Sulie ; Broschat, Kay O. ; Serif, George S. ; Ohio State Univ., Columbus</creatorcontrib><description>The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C
5 hydrogen has been established using GDP-
d-[5-
3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of
3H with the aqueous medium.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(85)90113-7</identifier><identifier>PMID: 2580455</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ALDEHYDES ; Analytical, structural and metabolic biochemistry ; AQUEOUS SOLUTIONS ; assays ; BASIC BIOLOGICAL SCIENCES ; BIOASSAY ; Biological and medical sciences ; CARBOHYDRATES ; CARBON-OXYGEN LYASES ; COMPARATIVE EVALUATIONS ; DISPERSIONS ; ENZYME ACTIVITY ; ENZYMES ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; GDP mannose 4,6-dehydratase ; Guanosine Diphosphate Mannose ; HEXOSES ; HYDRO-LYASES ; Hydro-Lyases - metabolism ; ISOTOPE APPLICATIONS ; LABELLED COMPOUNDS ; LYASES ; MANNOSE ; MIXTURES ; MONOSACCHARIDES ; ORGANIC COMPOUNDS ; SACCHARIDES ; SOLUTIONS ; SUBSTRATES ; TRACER TECHNIQUES ; Tritium ; TRITIUM COMPOUNDS ; Water</subject><ispartof>Anal. Biochem.; (United States), 1985, Vol.144 (1), p.253-257</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-1193e8ce4ff18f3c2ba213b7d89d1335390a2a0c858bd80bdca083e48a99c413</citedby><cites>FETCH-LOGICAL-c444t-1193e8ce4ff18f3c2ba213b7d89d1335390a2a0c858bd80bdca083e48a99c413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269785901137$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8482540$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2580455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5681701$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Sulie</creatorcontrib><creatorcontrib>Broschat, Kay O.</creatorcontrib><creatorcontrib>Serif, George S.</creatorcontrib><creatorcontrib>Ohio State Univ., Columbus</creatorcontrib><title>An assay for GDP- d-mannose-4,6-dehydratase</title><title>Anal. Biochem.; (United States)</title><addtitle>Anal Biochem</addtitle><description>The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C
5 hydrogen has been established using GDP-
d-[5-
3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of
3H with the aqueous medium.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ALDEHYDES</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>AQUEOUS SOLUTIONS</subject><subject>assays</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOASSAY</subject><subject>Biological and medical sciences</subject><subject>CARBOHYDRATES</subject><subject>CARBON-OXYGEN LYASES</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>DISPERSIONS</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GDP mannose 4,6-dehydratase</subject><subject>Guanosine Diphosphate Mannose</subject><subject>HEXOSES</subject><subject>HYDRO-LYASES</subject><subject>Hydro-Lyases - metabolism</subject><subject>ISOTOPE APPLICATIONS</subject><subject>LABELLED COMPOUNDS</subject><subject>LYASES</subject><subject>MANNOSE</subject><subject>MIXTURES</subject><subject>MONOSACCHARIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>SACCHARIDES</subject><subject>SOLUTIONS</subject><subject>SUBSTRATES</subject><subject>TRACER TECHNIQUES</subject><subject>Tritium</subject><subject>TRITIUM COMPOUNDS</subject><subject>Water</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFKAzEQhoMotVbfQKGIiKLRySbZTS5CqVqFgh56D9lklq60u5pshb69u7b0qKc5_N8M83-EnDK4Y8DSewDgNEl1dqXktQbGOM32SJ-BTilw0Pukv0MOyVGMH9BCQqY90kukAiFln9yMqqGN0a6HRR2Gk8d3OvR0aauqjkjFbUo9ztc-2MZGPCYHhV1EPNnOAZk9P83GL3T6Nnkdj6bUCSEaypjmqByKomCq4C7JbcJ4nnmlPeNccg02seCUVLlXkHtnQXEUymrtBOMDcr45W8emNNGVDbq5q6sKXWNkqlgGHXS5gT5D_bXC2JhlGR0uFrbCehVNlkLGNPB_QSZAZwlPW1BsQBfqGAMW5jOUSxvWhoHphJvOpulsGiXNr3CTtWtn2_urfIl-t7Q13OYX29xGZxdFsJUr4w5TQiVSQIs9bDBsxX6XGLriWDn0Zeh6-7r8-48fFymXuQ</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Chang, Sulie</creator><creator>Broschat, Kay O.</creator><creator>Serif, George S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1985</creationdate><title>An assay for GDP- d-mannose-4,6-dehydratase</title><author>Chang, Sulie ; Broschat, Kay O. ; Serif, George S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-1193e8ce4ff18f3c2ba213b7d89d1335390a2a0c858bd80bdca083e48a99c413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ALDEHYDES</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>AQUEOUS SOLUTIONS</topic><topic>assays</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOASSAY</topic><topic>Biological and medical sciences</topic><topic>CARBOHYDRATES</topic><topic>CARBON-OXYGEN LYASES</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>DISPERSIONS</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GDP mannose 4,6-dehydratase</topic><topic>Guanosine Diphosphate Mannose</topic><topic>HEXOSES</topic><topic>HYDRO-LYASES</topic><topic>Hydro-Lyases - metabolism</topic><topic>ISOTOPE APPLICATIONS</topic><topic>LABELLED COMPOUNDS</topic><topic>LYASES</topic><topic>MANNOSE</topic><topic>MIXTURES</topic><topic>MONOSACCHARIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>SACCHARIDES</topic><topic>SOLUTIONS</topic><topic>SUBSTRATES</topic><topic>TRACER TECHNIQUES</topic><topic>Tritium</topic><topic>TRITIUM COMPOUNDS</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Sulie</creatorcontrib><creatorcontrib>Broschat, Kay O.</creatorcontrib><creatorcontrib>Serif, George S.</creatorcontrib><creatorcontrib>Ohio State Univ., Columbus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Anal. Biochem.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Sulie</au><au>Broschat, Kay O.</au><au>Serif, George S.</au><aucorp>Ohio State Univ., Columbus</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An assay for GDP- d-mannose-4,6-dehydratase</atitle><jtitle>Anal. Biochem.; (United States)</jtitle><addtitle>Anal Biochem</addtitle><date>1985</date><risdate>1985</risdate><volume>144</volume><issue>1</issue><spage>253</spage><epage>257</epage><pages>253-257</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C
5 hydrogen has been established using GDP-
d-[5-
3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of
3H with the aqueous medium.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2580455</pmid><doi>10.1016/0003-2697(85)90113-7</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Anal. Biochem.; (United States), 1985, Vol.144 (1), p.253-257 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76071903 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 550201 - Biochemistry- Tracer Techniques ALDEHYDES Analytical, structural and metabolic biochemistry AQUEOUS SOLUTIONS assays BASIC BIOLOGICAL SCIENCES BIOASSAY Biological and medical sciences CARBOHYDRATES CARBON-OXYGEN LYASES COMPARATIVE EVALUATIONS DISPERSIONS ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology GDP mannose 4,6-dehydratase Guanosine Diphosphate Mannose HEXOSES HYDRO-LYASES Hydro-Lyases - metabolism ISOTOPE APPLICATIONS LABELLED COMPOUNDS LYASES MANNOSE MIXTURES MONOSACCHARIDES ORGANIC COMPOUNDS SACCHARIDES SOLUTIONS SUBSTRATES TRACER TECHNIQUES Tritium TRITIUM COMPOUNDS Water |
| title | An assay for GDP- d-mannose-4,6-dehydratase |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T07%3A23%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20assay%20for%20GDP-%20d-mannose-4,6-dehydratase&rft.jtitle=Anal.%20Biochem.;%20(United%20States)&rft.au=Chang,%20Sulie&rft.aucorp=Ohio%20State%20Univ.,%20Columbus&rft.date=1985&rft.volume=144&rft.issue=1&rft.spage=253&rft.epage=257&rft.pages=253-257&rft.issn=0003-2697&rft.eissn=1096-0309&rft.coden=ANBCA2&rft_id=info:doi/10.1016/0003-2697(85)90113-7&rft_dat=%3Cproquest_osti_%3E76071903%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14097236&rft_id=info:pmid/2580455&rft_els_id=0003269785901137&rfr_iscdi=true |