An assay for GDP- d-mannose-4,6-dehydratase

The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C 5 hydrogen has been established using GDP- d-[5- 3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.

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Bibliographische Detailangaben
Veröffentlicht in:	Anal. Biochem.; (United States) 1985, Vol.144 (1), p.253-257
Hauptverfasser:	Chang, Sulie, Broschat, Kay O., Serif, George S.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ALDEHYDES Analytical, structural and metabolic biochemistry AQUEOUS SOLUTIONS assays BASIC BIOLOGICAL SCIENCES BIOASSAY Biological and medical sciences CARBOHYDRATES CARBON-OXYGEN LYASES COMPARATIVE EVALUATIONS DISPERSIONS ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology GDP mannose 4,6-dehydratase Guanosine Diphosphate Mannose HEXOSES HYDRO-LYASES Hydro-Lyases - metabolism ISOTOPE APPLICATIONS LABELLED COMPOUNDS LYASES MANNOSE MIXTURES MONOSACCHARIDES ORGANIC COMPOUNDS SACCHARIDES SOLUTIONS SUBSTRATES TRACER TECHNIQUES Tritium TRITIUM COMPOUNDS Water
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Beschreibung
Zusammenfassung:	The mechanism of GDP-D-mannose-4,6-dehydratase action with respect to loss of the C 5 hydrogen has been established using GDP- d-[5- 3H]-mannose as a substrate. This observation has been incorporated into a rapid assay for the enzyme based on the equilibration of 3H with the aqueous medium.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(85)90113-7