Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene

A 1.2-kilobase (kb) HindIII restriction fragment containing the pilin gene from Pseudomonas aeruginosa PAK has been cloned and sequenced. The pilin protein is 144 amino acids in length with a positively charged leader sequence of 6 amino acids. There is probably only one copy of the gene per chromos...

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Veröffentlicht in:	FEBS letters 1985-04, Vol.183 (2), p.413-416
Hauptverfasser:	Pasloske, Brittan L., Finlay, B.Brett, Paranchych, William
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacterial Outer Membrane Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Chromosome location Cloning, Molecular Deoxyribonuclease HindIII DNA Restriction Enzymes - metabolism DNA sequence Electrophoresis, Agar Gel Fimbriae Proteins Fundamental and applied biological sciences. Psychology Genetics Leader cleavage Leader sequence Microbiology Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa pilin gene
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Beschreibung
Zusammenfassung:	A 1.2-kilobase (kb) HindIII restriction fragment containing the pilin gene from Pseudomonas aeruginosa PAK has been cloned and sequenced. The pilin protein is 144 amino acids in length with a positively charged leader sequence of 6 amino acids. There is probably only one copy of the gene per chromosome.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80821-8