Analysis of active antibody concentration. Separation of affinity and concentration parameters
Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independen...
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| Veröffentlicht in: | Journal of immunological methods 1993-11, Vol.166 (1), p.75-84 |
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| container_end_page | 84 |
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| container_issue | 1 |
| container_start_page | 75 |
| container_title | Journal of immunological methods |
| container_volume | 166 |
| creator | Karlsson, Robert Fägerstam, Lars Nilshans, Helena Persson, Björn |
| description | Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity. One antibody calibration curve covering the range from 0.5 to 160 nM (0.08–25 μg/ml) antibody was valid for IgG antibodies with different antigen specificities. To illustrate the use of this methodology active antibody concentrations were analysed in culture media and in rabbit serum. |
| doi_str_mv | 10.1016/0022-1759(93)90330-A |
| format | Article |
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To illustrate the use of this methodology active antibody concentrations were analysed in culture media and in rabbit serum.</description><subject>Affinity</subject><subject>Animals</subject><subject>Antibodies - analysis</subject><subject>Antibodies - blood</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody</subject><subject>Antibody Affinity</subject><subject>Antigen-Antibody Reactions</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Concentration</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. 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Separation of affinity and concentration parameters</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1993-11-05</date><risdate>1993</risdate><volume>166</volume><issue>1</issue><spage>75</spage><epage>84</epage><pages>75-84</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><coden>JIMMBG</coden><abstract>Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity. One antibody calibration curve covering the range from 0.5 to 160 nM (0.08–25 μg/ml) antibody was valid for IgG antibodies with different antigen specificities. 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| ispartof | Journal of immunological methods, 1993-11, Vol.166 (1), p.75-84 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Affinity Animals Antibodies - analysis Antibodies - blood Antibodies, Monoclonal Antibody Antibody Affinity Antigen-Antibody Reactions Biological and medical sciences Biosensing Techniques Concentration Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Fundamental immunology Immunoassay - methods Kinetics Mass transfer Molecular immunology Rabbits Techniques Theophylline - immunology |
| title | Analysis of active antibody concentration. Separation of affinity and concentration parameters |
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