Analysis of active antibody concentration. Separation of affinity and concentration parameters

Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independen...

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Veröffentlicht in:	Journal of immunological methods 1993-11, Vol.166 (1), p.75-84
Hauptverfasser:	Karlsson, Robert, Fägerstam, Lars, Nilshans, Helena, Persson, Björn
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Animals Antibodies - analysis Antibodies - blood Antibodies, Monoclonal Antibody Antibody Affinity Antigen-Antibody Reactions Biological and medical sciences Biosensing Techniques Concentration Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Fundamental immunology Immunoassay - methods Kinetics Mass transfer Molecular immunology Rabbits Techniques Theophylline - immunology
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Beschreibung
Zusammenfassung:	Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity. One antibody calibration curve covering the range from 0.5 to 160 nM (0.08–25 μg/ml) antibody was valid for IgG antibodies with different antigen specificities. To illustrate the use of this methodology active antibody concentrations were analysed in culture media and in rabbit serum.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(93)90330-A